Illumina HiSeq 2500 paired end sequencing; GSM3124789: Sample_10 [RNA-seq]; Homo sapiens; RNA-Seq

Sample_10 [RNA-seq]

Maternal plasama colected longitudinally were profiled using paired-end Illumina RNA-Seq with globin reduction to evaluate changes with gestational age and with labor in normal pregnancy. Overall design: The study included normal pregnancies with (n=8) and without (n=8) spontaneous labor at term.  Half of the women in each group had 3 longitudinal samples taken from 12.1-40.3 weeks of gestation, while the other half of women had only one sample taken at term before delivery, for a total of 32 samples.

Sequencing based maternal whole blood expression changes with gestational age and labor in normal pregnancy

Submitted by Gene Expression Omnibus on 09-FEB-2019

RNA was isolated from  PAXgene® Blood RNA collection tube (BD 762165) as described in the PAXgene® Blood miRNA Kit Handbook (December, 2015). Purified RNA was quantified by UV spectrophotometry using the DropSense96® Microplate Spectrophotometer (Trinean) and quality assessed by microfluidics using the RNA ScreenTape on the Agilent 2200 TapeStation. Starting with 500 ng of total RNA, cDNA library templates were synthesized using the Illumina TruSeq® Stranded Total RNA LT (Set A) Kit with Ribo-Zero Globin rRNA reduction, as described in the TruSeq® Stranded Total RNA Sample Preparation Guide (Rev. E, October 2013). Libraries were validated using the HS D1000 ScreenTape on the Agilent 2200 TapeStation, and quantified on the Qubit® 2.0 Fluorometer by the Qubit® dsDNA HS Assay (ThermoFisher). The Illumina HiSeq 2500 instrument was used to cluster the samples onto the flow cell. The pools were put on at 10pM and run in rapid mode at 2×100 paired end. Illumina HiSeq Rapid PE Cluster kit V2 and HiSeq Rapid SBS kit v2 200 cycles was used.

Targeted expression profiling by RNA-Seq improves detection of cellular dynamics during pregnancy and identifies a role for T cells in term parturition.

Sequencing based maternal whole blood expression changes with gestational age and labor in normal pregnancy

Submitter Supplied Information

Samples