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Accession IconSRP142340

Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [RNA-Seq]

Organism Icon Oryza sativa, Nilaparvata lugens
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Technology Badge IconIllumina HiSeq 2500

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research was focus on the virus-derived sRNAs expression pattern in rice host or planthopper vector and host/vector gene expression pattern when infected with RSV Overall design: The viruliferous and non-viruliferous small brown planthopper strains used in this work were derived from a rice field population in Hai'an, Jiangsu Province, China, and maintained in the laboratory for nearly nine years. The planthoppers were reared on 2 cm to 3 cm seedlings of rice (Oryza sativa Huangjinqing) in glass incubators, which were sealed with a nylon mesh, at 25 ± 1°C, 80% relative humidity, and 16 h of light per day. The planthoppers were transferred to fresh rice seedlings every 8 days to ensure sufficient nutrition.
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