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Accession IconSRP140739

Phenotyping spermatogenic defects by single-cell expression profiling

Organism Icon Mus musculus
Sample Icon 24 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500, Illumina MiSeq, Illumina HiSeq 3000

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Description
Using Drop-seq, we generated high-throughput single-cell expression data from wild-type and four mutant models with male infertility phenotype. Our study demonstrates the applicability of single-cell RNA-sequencing in study of male gonadal dysfunction and provides cell atlas resource for testis. Overall design: Drop-seq was performed on FACS sorted germ cell populations, wild-type whole testes and mutant whole testes. Different experimental batches for wild-type and mutant strains were generated.
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30
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No associated institution
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