Transcriptomic analysis of myosin IIa-deficient B cells

SRP139927

Mus musculus

6 Downloadable Samples

Illumina HiSeq 2500

Submitter Supplied Information

Description

Myosin IIa-deficient follicular B cells have a hyperactivated phenotype. To identify what pathways are regulated by myosin IIa, we performed RNA-seq of coding RNA on flow cytometry sorted follicular B cells from CD23Cre+Myh9fl/fl and CD23Cre+Myh9wt/fl mice. Overall design: B220+AA4.1-CD23+CD21lo follicular B cells were sorted from 3 CD23Cre+Myh9fl/fl and 3 CD23Cre+Myh9wt/fl mice and mRNA was isolated and sequenced.

PubMed ID

29791846

Publication Title

Myosin IIa Promotes Antibody Responses by Regulating B Cell Activation, Acquisition of Antigen, and Proliferation.

Total Samples

Submitter’s Institution

No associated institution

Authors

Hoogeboom R, Natkanski EM, Nowosad CR, Malinova D, Menon RP, Casal A, Tolar P

Source Repository

Sequence Read Archive (SRA)

Alternate Accession IDs

GSE113114

No rows found