Temporal profiling of redox-dependent heterogeneity in single cells

The cellular redox status plays an essential role in diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus even cells with identical genetic background but different redox status behave differently. Here, we propose a non-laborious and robust methodology to quantify cell-to- cell redox-dependent variability within thousands of cells, detect oxidation levels in cytosol, mitochondria, and peroxisome, and sort cells based on their redox status. We report that cells of the same age have a bi-modular distribution of reduced and oxidized cells starting in the late logarithmic phase. Although the ratio between the oxidized and reduced subpopulations changes during chronological aging, their growth, proteomic and transcriptomic features are conserved during their first days of chronological aging. A comparative proteomic analysis identified three key proteins, Hsp30, Dhh1, and Pnc1, which affect basal oxidation levels and may serve as first line of defense proteins in redox homeostasis. Overall design: The dataset includes 12 3''RNA-Seq samples.

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Radzinski M, Fassler R, Yogev O, Breuer W, Shai N, Gutin J, Ilyas S, Geffen Y, Tsytkin-Kirschenzweig S, Nahmias Y, Ravid T, Friedman N, Schuldiner M, Reichmann D