THE DNA BINDING LANDSCAPE OF THE MAIZE AUXIN RESPONSE FACTOR FAMILY [RNA-seq]

DAP-seq was used to generate genome-wide DNA:TF interaction maps for fourteen maize ARFs from the evolutionarily conserved class A 'activator' and class B 'repressor' clades. Among the distinct binding sites that were identified, we observed a high degree of overlap for ARFs of the same class, but found substantial differences in motif sequence, spacing, site preference, and association with auxin induced genes among clade A and clade B ARFs. Overall design: RNA-seq was performed on young maize seedlings with and without exogenous application of 100mM IAA for 30 min.

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Galli M, Khakhar A, Lu Z, Chen Z, Sen S, Joshi T, Nemhauser JL, Schmitz RJ, Gallavotti A