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Accession IconSRP132174

Transcriptome response study of arabidopsis wild type (Col6-3), gun1-9 mutant and MORF2 overexpression lines to norflurazon treatment in retrograde signaling

Organism Icon Arabidopsis thaliana
Sample Icon No Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Analysis of transcriptome response of norflurazon treated or untreated 5-day old whole seedlings with genotypes: Col6-3 (wild type), gun1-9 and MORF2 overexpression lines. GUN1 and MORF2 are involved in chloroplast-to-nucleus retrograde signaling. Results provide insight into the nuclear genes expression profile under control of GUN1 retrograde pathways and the regulation similarity between gun1-9 and MORF2 overexpression lines. Overall design: Total RNA samples were isolated from 5-day old whole seedlings with genotypes Col6-3 (wild type), gun1-9 and MORF2 overexpression lines grew under 24 h light condition with 100 µmol.m-2.s-1 light intensity at 22 °C with or without 5 µM norflurazon treatment. MORF2OX and MORF2OX(w) are both overexpression lines of MORF2 but they have different strengths of the phenotype. mRNA expression profiles were generated by deep sequencing, in duplicate, using Illumina HiSeq 2500.
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