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Accession IconSRP131201

Pathogen Trojan horse delivers bioactive host protein ZmMAC1 to alter maize (Zea mays) anther cell behavior in the male sterile mutant Zmmac1-1.

Organism Icon Zea mays
Sample Icon No Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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To determine if TH delivery of ZmMAC1 protein results not only in cytological but also molecular cell differentiation in Zmmac1-1, gene expression was determined using RNA sequencing of the infected anthers. Overall design: There were four biological replicates of fertile anther infected with SG200 (F_SG200a/b/c/d) as control to show normal somatic niche and AR cell progression; two biologcial replicates of Zmmac1-1 sterile anthers infected with SG200 (mac1_SG200a/b) as negative controls lacking the somatic niche and overproducing AR cells; and four biological replicates of Zmmac1-1 sterile anthers infected with SG200 carrying ZmMAC1.
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