github link
Accession IconSRP127728

RNA-seq sequencing of the transgenic plants seeds

Organism Icon Zea mays
Sample Icon No Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

Submitter Supplied Information

Description
To analyze influence of ZmDWF4 on seed development, we we chose 10DAP and 15DAP from the transgenic line L100 seed to RNA-seq sequence. Total RNA was extracted from the seeds (10 days after pollination, 10DAP, 15 days after of pollination,15DAP) of transgenic line L100 and wild type Q319. cDNA library was builded in the BIOMARKER, original reads were sequenced based on Illumina HiSeq2000, and through rRNA, low quality fragment DNA of filtering, ultimately, we obtained 99.30Gb high quality clean reads. Compared with reference gene sequence(AGPv3), according to genes function annotation, we found 463, 675 different expression genes, respectively.We classified the different expression genes according to Cellular Component, Molecular Function and Biological Process. It showed that BRs could affect many genes expression involved in different biological process in the seed development. Overall design: Examination of the 10DAP and 15DAP seeds from transgenic line L100 and wild type Q319
PubMed ID
No associated PubMed ID
Publication Title
No associated publication
Total Samples
12
Submitter’s Institution
No associated institution
Authors
No associated authors
Alternate Accession IDs

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Specimen part
Subject
Processing Information
Additional Metadata
No rows found
Loading...