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Accession IconSRP127392

The chromatin remodeler Bptf activates a stemness gene-expression program essential for the maintenance of adult hematopoietic stem cells [RNA-Seq]

Organism Icon Mus musculus
Sample Icon 4 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000, Illumina HiSeq 2500

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Bptf, a component of NURF chromatin-remodeling complex, is essential for maintaining the pool size and function of hematopoietic stem cells (HSCs). Genome-wide transcriptome profiling revealed that Bptf loss caused down-regulation of HSC-specific gene-expression programs, which included master transcription factors (such as Meis1, Pbx1, and Lmo2) known to be required for HSC maintenance and self-renewal. Bptf directly bound to the promoter of 'stemness' TF genes, potentiating their transcription and DNA accessibility. Overall design: To dissect the gene-regulatory role of Bptf in HSPCs, we sorted out phenotypically identical LSK cells from the bone marrow of Bptf conditional KO mice and littermate controls, followed by ACAT-seq and RNA-seq studies. We also performed H3K4me3 ChIP-seq using HPC7 cells, a murine HSPC-mimicking cell line.
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