Transcriptomics of Dorso-Ventral axis determination in Xenopus tropicalis

We studied the transcriptomic differences between ventralised and dorsalised Xenopus tropicalisembryos as a result of UV irradiation and LiCl treatment, respectvely. Both manipulations have been used by researchers to perturb the establishment of the dorso-ventral axis, however the whole transcriptomes of the resulting embryos have never been fully characterised or compared. We show that ventralized embryos are transcriptionally closer related to untreated embryos than dorsalized embryos. Furthermore comparison to dissected ventral and dorsal parts of an unperturbed gastrula embryo indicates that UV and LiCl treatment indeed enriches for ventral and dorsal cells, respectively. Overall design: Comparison of poly(A) transcriptome between Xenopus tropicalis LiCl-, UV-treated and untreated gastrulae embryos. For each condition there are five biological replicates that were used for the analyses.

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Monteiro RS, Gentsch GE, Smith JC