RNA-seq of WT, hrr25as, and hrr25is treated with inhibitors

The carboxyl-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) orchestrates dynamic recruitment of specific cellular machines during different stages of transcrption. Signature phosphorylation patterns of Y1S2P3T4S5P6S7 heptapeptide repeats of the CTD engage specific "readers." While phospho-Ser5 and phospho-Ser2 marks are ubiquitous, phospho-Thr4 is reported to only impact specific genes. Here, we investigate the transcriptome in WT cells, hrr25as cells, or hrr25is cells in the presence or absence of ATP analog inhibitors. Overall design: Total RNA was collected from WT, hrr25as, and hrr25is cells treated with either DMSO or CMK.

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Nemec CM, Singh AK, Ali A, Tseng SC, Syal K, Ringelberg KJ, Ho YH, Hintermair C, Ahmad MF, Kar RK, Gasch AP, Akhtar MS, Eick D, Ansari AZ