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Accession IconSRP125035

Gene expression profiling of mouse macrophages following long noncoding RNA Malat1 knockdown

Organism Icon Mus musculus
Sample Icon 4 Downloadable Samples
Technology Badge IconNextSeq 500

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We applied next-generation sequencing to investigate the gene expression profiles in mouse bone-marrow derived macrophages with or without long noncoding RNA-Malat1 knockdown. We identified a number of differentially regulated genes in cells with Malat1 knockdown. Overall design: Mouse bone-marrow derived macrophages were transfected with Antisense LNAâ„¢ GapmeRs against Malat1 or Negative Control oligos (Exiqon). 48h after transfection, total RNA was isolated and subjected to high-throughput sequencin (RNA-seq), using Illumina GAIIx. Gene expression profiles were compared between two groups.
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