Identification of immune-activated Hematopoietic Stem Cells

We utilized our transgenic Fgd5-mCherry mouse to sort and RNAseq for HSCs under acute immune activation (with pIC) to reveal a complex cell cycle gene expression and an upregulated IFN I/II signature Overall design: RNAseq of bone marrow Lineage-Sca1+cKit+CD150+mCherry+ cells (1000) 24hrs after pIC was administered and control (PBS treated)

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Bujanover N, Goldstein O, Greenshpan Y, Turgeman H, Klainberger A, Scharff Y, Gazit R