High-throughput deep sequence of zebrafish wild-type and ezh2 mutant embryos

To determine the molecular basis for the reduced blood cells in circulation in ezh2 mutant zebrafish, we performed high-throughput sequencing at 28 hpf. Results revealed 893 up-regulated genes and 425 down-regulated genes in the ezh2 mutant zebrafish compared with wild-type zebrafish. We found that some hematopoietic genes are down-regulated in ezh2 mutant zebrafish, which was reconfirmed with qRT-PCR. Overall design: mRNA profiles of 28 hpf wild-type and ezh2 mutant zebrafish were generated by deep sequncing.

2

Zhong Y, Ye Q, Chen C, Wang M, Wang H