Illumina HiSeq 2500 paired end sequencing; GSM2784988: H1TPSC1; Homo sapiens; RNA-Seq

H1TPSC1

Combined transcriptomic and functional analyses of HCC cells at single-cell level were performed to assess CSC heterogeneity. Overall design: Single-cell transcriptome analyses of two HCC cell lines (HuH-1 and HuH-7) and one patient-derived circulating tumor cells by using SMART-Seq protocol ***Due to patient privacy concerns, the submitter declares that patient data will be submitted to dbGaP.***

Single cell analysis reveals cancer cell heterogeneities in hepatocellular carcinoma [SMART-seq]

Submitted by Gene Expression Omnibus on 13-JUN-2018

no RNA extraction step droplet-based single-cell RNA-seq (SMART-seq) Cells were lysed in ice-cold lysis buffer. SMART-seq protolcol was performed on single or pooled sorted cells following the manufacturere's instructions with some modification. Briefly, whole transcriptome was amplified with the SMART-seq v4 Ultra Low Input RNA kit (Clonetech), purified (Beckman Coulter, A63881) and barcoded (Illumina, FC-131-1096). Finally, libraries were pooled and sequenced on Illumina Hiseq2500.

Single cell analysis reveals cancer cell heterogeneities in hepatocellular carcinoma [SMART-seq]

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Samples