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Accession IconSRP115928

Genome-wide analyses of direct target genes of four rice NAC-domain transcription factors involved in drought tolerance [RNA-seq]

Organism Icon Oryza sativa
Sample Icon No Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
To understand the molecular mechanisms of drought tolerance, we performed ChIP-Seq and RNA-Seq analyses to identify direct target genes of the OsNACs using the RCc3:MYC-OsNACs roots. A total of 475 binding loci of 4 OsNACs were identified by cross-referencing the binding occupancy of OsNACs at promoter regions and expression levels of corresponding genes. The binding loci are distributed on promoter regions of 391 target genes that were directly up-regulated by OsNACs in four RCc3:MYC-OsNAC transgenic roots. The direct target genes were related to transmembrane/transporter activity, vesicle, plant hormone, carbohydrate metabolism, and transcription factors. The direct targets of each OsNAC are in a range of 4.0 to 8.7% of the genes up-regulated in RNA-Seq data sets. Thus, each OsNAC up-regulates of corresponding direct target genes that alters root system architectures of RCc3:OsNACs for drought tolerance. Overall design: RNA-seq using roots of NT (nontransgenic plants), RCc3:myc-OsNAC5, 6, 9, and 10 transgenic plants that were generated by deep sequencing using Illumina Hiseq 2500.
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10
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