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Accession IconSRP113651

Arabidopsis thaliana Transcriptome or Gene expression

Organism Icon Arabidopsis thaliana
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Technology Badge IconIllumina HiSeq 2000

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Ten-day-old seedlings were dehydrated on dry filter paper in Petri dishes till loss of 40% fresh weight and then incubated for 2 h in sealed plastic bags to prevent further water loss. Total RNA was extracted using the RNeasy mini kit (Invitrogen), and DNA was cleaned by DNase I (New England Biolabs). About 2 to 4 µg of cleaned total RNA was used to construct RNA-seq libraries by using an Illumina Whole Transcriptome Analysis Kit following the standard protocol (Illumina, HiSeq system) and sequenced on the HiSeq 2000 platform.
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8
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