Polymerases IV and V affect Pol II transcription termination

Nuclear run-on assays reveal that in the absence of Pol IV or Pol V, Pol II occupancy downstream of poly A sites increases for approximately 12% of protein-coding genes. Overall design: Analysis of nascent and steady-state RNA levels in pol IV (nrpd1-3) and pol V (nrpe1-11) mutant plants

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McKinlay A, Podicheti R, Wendte JM, Cocklin R, Rusch DB