RNA-Seq expression profiling of mouse DEN-initiated hepatocytes with or without Tgfbr2 inactivation

Initiated hepatocytes (IHCs), isolated from DEN exposed Tgfbr2 flox/flox mouse, were infected with GFP or Cre-expressed adenovirus, followed by 1ng/ml TGFß × 48 hours incubation. Then total RNAs were isolated and processed to routine sequencing on Illumina platform. RNA-Seq libraries were prepared from total RNA using polyA enrichment. Overall design: Examination of transcriptome differences between initiated hepatocytes with or without Tgfbr2 inactivation.

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Zhang J, Han C, Ungerleider N, Chen W, Song K, Wang Y, Kwon H, Ma W, Wu T