Genome-wide analysis of heat effect on rice transcriptome by RNA-seq

To impose the heat treatment similar to the Structure-seq experiment, we applied RNA-seq to 14-day-old rice (Oryza sativa) shoot tissue to investigate the effect of heat stress (42°C) as compared to control condition (22°C). Overall design: Two week old rice plants in pots were inverted and the shoots were immersed in a water bath at 22°C or 42°C for 10 min treatment, and the plants were then transferred to a growth chamber set at the same temperature as in the greenhouse (30°C) for ease of sampling during the recovery period (0 min, 10 min, 50 min, 1 h 50 min, 9 h 50min). Three rice shoots comprised one biological replicate, and two biological replicates were obtained for each treatment and time-point.

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Su Z, Tang Y, Ritchey LE, Tack DC, Zhu M, Bevilacqua PC, Assmann SM