NextSeq 500 paired end sequencing; GSM2689401: e14.5 EP 1; Mus musculus; RNA-Seq

e14.5 EP 1

MATQ-sequencing of single isolated endocrine progenitors (EPs) from the e14.5 and e16.5 mouse pancreas Overall design: MATQ-seq from two litters of Ngn3-eGFP e14.5 and two litters of e16.5 mice, with 15 cells from e14.5 and 12 cells from e16.5. sequenced in two batches of library prep and sequencing

High-Resolution temporal profiling of developing pancreas uncovers the mechanisms of endocrine cell fate determination [MATQ-seq]

Submitted by Gene Expression Omnibus on 07-JUN-2018

After flow sorting, Ngn3-eGFP+ cells were collected in RPMI medium. We used mouth pipette to pick single cells into PCR tubes with MATQ-seq lysis buffer (Sheng et al., 2017). MATQ-seq was then performed to amplify the whole transcriptome of single cells. After second strand synthesis, the input and yield of each sample was quantified by qPCR. Highly degraded samples or samples with multiple cells were discarded. After PCR amplification, we performed library preparation and data analysis according to the previous publication (Sheng et al., 2017). Duplex specific Nuclease was used to remove the majority of ribosomal cDNA.

High-Resolution temporal profiling of developing pancreas uncovers the mechanisms of endocrine cell fate determination [MATQ-seq]

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