Polytrichastrum alpinum is one of the moss species that survives extreme conditions in Antarctic. In order to explore the function of P. alpinum multiprotein-bridging factor 1c gene (PaMBF1c), the gene was overexpressed in Arabidopsis and the overexpression phenotypes were analyzed by comparing with the wildtype and/or the Arabidopsis own MBF1c (AtMBF1c) overexpressors. Overexpression of PaMBF1c in Arabidopsis resulted in enhanced tolerance in salt, osmotic, and cold stresses as well as heat stress. More specifically, PaMBF1c overexpressing lines showed better salt tolerance than the wild type, which was not clearly observed in the AtMBF1c overexpressing lines. To investigate these phenotype differences in wild-type Arabidopsis, PaMBF1c overexpressor, and AtMBF1c overexpressors, RNA-seq analysis was performed for these three different plants under normal condition and salt stress.