Illumina HiSeq 2500 paired end sequencing; GSM2651508: IA3023_RNA-seq; Glycine max; RNA-Seq

IA3023_RNA-seq

A comparison of RNA expression profiles across a diverse set of soybean genotypes Overall design: RNA-seq of 41 soyNAM parental lines and 61 milestone cultivars

Transcriptomes of 102 soybean accessions

Submitted by Gene Expression Omnibus on 09-JUN-2017

Tissue was flash frozen in liquid nitrogen and RNA was isolated using TRIzol (Thermo Scientific, Waltham, MA) following the manufacturer’s instructions. RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA) following the manufacturer’s instructions with limited modifications. The starting quantity of total RNA was adjusted to 1.3 µg, and all volumes were reduced to a third of the described quantity.

Transcriptomes of 102 soybean accessions

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Samples