RNASequencing of nf-yc, nf-yb, and CO-SAIL mutants during photomorphogenic growth in Arabidopsis thaliana. (thale cress)

The purpose of this RNA Sequencing project was to investigate the transcriptional regulatory relationship between NF-YC3/4/9, NF-YB2/B3 and CO-SAIL. Methods: Total RNA was isolated, and then poly-A purified. 100ng of starting RNA was used to generate RNASeq libraries using the NEXTflex Illumina qRNA-Seq Library Prep Kit, and sequenced on an Illumina HiSeq2500 machine. Results: NF-YC3/4/9, NF-YB2/B3 and CO-SAIL have both shared and independent regulatory targets. Overall design: mRNA profiles of 7-day old Wild Type (WT), nf-yc triple (Q3), nf-yb3/b3, and CO-SAIL seedlings grown in continuous white light were generated from two biological replicates through paired end deep sequencing on an Illumina HiSeq2500 platform.

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