Total RNA was extracted from gluteus medius muscle samples by using the acid phenol method implemented in the RiboPure kit (Ambion, Austin, TX). Total RNA concentration was assessed with a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Rockford, IL, USA), while purity and integrity were checked with a Bioanalyzer-2100 equipment (Agilent Technologies, Inc., Santa Clara, CA). Individual libraries for each one of the analysed pigs (N = 36) were prepared using the TruSeq RNA Sample Preparation Kit (Illumina Inc., CA) according to the protocols recommended by the manufacturer. Each library was paired-end sequenced (2 x 75 bp) in a HiSeq 2000 platform (Illumina Inc., CA) by using the TruSeq SBS Kit v3-HS (Illumina Inc., CA). Quality control was carried out with FASTQC to confirm the quality of sequence reads. The Sscrofa10.2 reference genome index (Sscrofa10.2, http://www.ensembl.org/info/data/ftp/index.html) was built using the Bowtie2-build component of Bowtie2 v.2.2.3 and  TopHat Alignment Tool v.2.0.9 was use to mapping.

Illumina HiSeq 2000 paired end sequencing; pre- and post-prandial muscle transcriptomes of Duroc pigs.

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Genomic physiology of intramuscular fat storage in pigs

Sus scrofa Transcriptome or Gene expression

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Samples