Illumina HiSeq 2000 sequencing; GSM2631429: Brain astrocyte single cell 1; Mus musculus; RNA-Seq

Brain astrocyte single cell 1

By analyzing 250 astrocyte single cell transcriptomes from adult brain, we provide gene expresssion profile of brain astrocyte Overall design: We chose adult mice about 3 months old and analysed single cells in the brain. We chose a methodology based on fluorescence-activated cell sorting (FACS) into 384-well plates followed by the SmartSeq2 methodology.

Single cell RNA-seq of mouse brain astrocyte transcriptomes

Submitted on 08-DEC-2017

We used transgenic mouse reporter line (SM22-Cre;Rosa26-stop-tdTomato). We chosed to focus our efforts to adult mice brain cells and we chose a methodology based on fluorescence-activated cell sorting (FACS) into 384-well plates followed by the SmartSeq2 methodology for the generation and sequencing of full length polyadenylated cDNAs Libraries for RNA-seq were prepared by using SMART-seq2 methodology

Single cell RNA-seq of mouse brain astrocyte transcriptomes

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