Illumina HiSeq 2000 paired end sequencing; GSM2598175: SD_Wk2 post-sham_RNASeq_rep1; Rattus norvegicus; RNA-Seq

SD_Wk2 post-sham_RNASeq_rep1

We report the changes in left ventricle mRNA abundance in response to 5/6 nephrectomy surgery Overall design: Ten week old male Sprague Dawley rats were subjected to the excision model of 5/6 nephrectomy (5/6Nx) or sham surgery. Left ventricle tissue was collected 2, 4, 5 or 7 weeks later for mRNAsequencing.

Molecular Mediators of Cardiac Pathology in Cardiorenal Syndrome Type 4 [mRNA]

Submitted by Gene Expression Omnibus on 05-MAY-2017

Left ventricle tissues were isolated and flash frozen in liquid nitrogen.   They were then ground into powder under liquid nitrogen which was thawed in Trizol for chloroform-based RNA isolation. mRNA libraries were constructed for these pools using the Illumina TruSeq Stranded Total RNA Kit.   Samples were multiplexed seven per lane on a 300 Gb flow cell and paired-end sequenced with an Illumina HiSeq 2000.

Molecular Mediators of Cardiac Pathology in Cardiorenal Syndrome Type 4 [mRNA]

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