Gene expression analysis using RNA Sequencing of the Saccharomyces cerevisiae BY4741 Spt4-T42A/S43A mutant strain upon osmotic stress.

To study the relevance of the phosphorylation of Spt4 in residues T42 and S43, we assessed the genome-wide transcriptional response of the non-phosphorylatable Spt4-T42A/S43A mutant (Spt4-AA) upon osmotic stress. Results point out that the unphosphorylatable Spt4-T42A/S43A protein showed an impaired stress responsive gene expression indicating that these specific residues play a relevant role in osmotic stress responsive transcriptional regulation. Overall design: Total RNA obtained from the Saccharomyces cerevisiae BY4741 Spt4-TAP (WT) or BY4741 Spt4-T42A/S43A-TAP mutant (Spt4-AA) untreated or subjected to osmotic stress (15 min at 0.4M NaCl). Experiment was done by triplicate.

12

Silva A, Cavero S, Begley V, Solé C, Böttcher R, Chávez S, Posas F, de Nadal E