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Accession IconSRP103124

ARF5/MP responsive genes in the vascular cambium of Arabidopsis

Organism Icon Arabidopsis thaliana
Sample Icon 6 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
15-20 cm tall PXY:GR-MP?III/IV plants were dipped headfirst in 15 µM dexamethasone or mock solution and after three hours of incubation second internodes were harvested and snap frozen in liquid nitrogen. Frozen plant material was pulverized with pestle and mortar and RNA was isolated by phenol/chlorophorm extraction as described previously (Mallory & Vaucheret 2010, PlantCell) with the modification of two additional concluding 70% EtOH washes Overall design: RNA from three samples was pooled and analyzed by RNAseq.
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6
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No associated institution
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