Transcriptome analysis on wild type and ddx39a mutant zebrafish embryos by Next Generation Sequencing

DEAD box RNA helicase DDX39A has been shown to regulate RNA metabolism; however its role in vertebrate development has not previously been examined. To determine the impact of loss of ddx39a on transcriptome during vertebrate early development, we pursued transcriptome analysis (RNA-Seq) on wild type and ddx39a mutant zebrafish embryos at 24 hour-post-fertilization. And by using RIP-seq to identify targeted RNA which were DDX39A binded. Overall design: Using RNA-seq to identify the changes in the transcriptomic landscape in ddx39a mutants. Using RIP-seq to identify DDX39A binding RNAs.

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