Next Generation Sequencing analysis using splenic DCs freshly isolated from Wild Type and TBK1-DKO mice

In this experiment, we analyzed the gene expression profile of WT and TBK1-deficient splenic DCs by RNA sequencing based on three independent samples. The results revealed significant alterations in the expression of a number of genes, most notably the enhanced expression of a large subset of IFN-responsive genes in the TBK1-deficient DCs. Overall design: Fresh splenic DC mRNA profiles of 8-week old wild type (WT) and TBK1-DKO mice were generated by deep sequencing, in triplicate

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Xiao Y, Zou Q, Xie X, Liu T, Li HS, Jie Z, Jin J, Hu H, Manyam G, Zhang L, Cheng X, Wang H, Marie I, Levy DE, Watowich SS, Sun SC