Arabidopsis plants were grown in soil at 22¢XC under 70-125 £gE-2 sec-1 irradiance (16 h light and 8 h dark).Total RNA extracted from 21-day-old rosette leaves with PureLink Plant RNA Reagent (Thermo Fisher Scientific) was used in library construction for RNA-seq profiling. The RNA-seq libraries were prepared using the Illumina TruSeq RNA Library Preparation Kit v2 (http://www.illumina.com/).

Illumina HiSeq 2500 sequencing; RNA-Seq of Arabidopsis thaliana:rosette leaves of dcl4rdr6 mutant

Arabidopsis dcl4 rdr6 double mutant

Identification of genes silenced by the DCL2-RDR6 pathway in the dcl4 mutant plants producing purple leaves under a standard growthcondition.

Transcriptome and small RNA-Seq analysis of PTGS-defective Arabidopsis mutants

Submitted by ACADEMIA SINICA on 25-SEP-2017

DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation.