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Accession IconSRP097006

Distinct roles for matrix metalloproteinases 2 and 9 in hematopoietic stem cell emergence, migration and niche colonization

Organism Icon Danio rerio
Sample Icon 6 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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We report gene expression data for FACS sorted zebrafish mpeg1:mCherry + and mpx:EGFP + cells collected from whole embryos at 72 hours post fertilization (hpf). We also report gene expression data for the remaining, transgene negative, portion of these embryos. Overall design: ~1,000 mpeg1:mCherry+; mpx:EGFP+ transgenic embryos were homogenized, filtered, and sorted using FACS into PBS, collecting >50,000 cells for each of the three populations: mpeg1:mCherry+, mpx:EGFP+ and double negative (no double positive cells were collected as there was almost no overlap between mCherry and EGFP expression).
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