Analysis of transcriptome between clv3 and mpclv3

The targets of AUXIN REPONSE FACTOR5/MONOPTEROS (ARF5/MP) were reported in many studies which mainly concerned to the flower initiation. But most of these targets are expressed in periphery zone (PZ), how MP regulate the stem-cell activity is unclear. In order to specifically identify the targets of MP in stem cells, as clv3 mutant enrich lots of stem cells, thus we performed transcriptomes of clv3 and mpclv3. This method will enhance the resolution of MP targets specific in stem cells. And it will also avoid the severe morphologic difference between WT and mp single mutant. Overall design: Examination of transcriptome profile in 2 genotypes with 2 biological replicates each.

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Luo L, Zeng J, Wu H, Tian Z, Zhao Z