Bombyx mori Argonaute2 associated long RNAs (domestic silkworm)

Argonaute (AGO) proteins mediate interaction of incorporated small RNAs with their targets. The resolution of AGO associated small RNAs and their targets showed a significant landscape of AGO proteins and their binding RNAs. Previously, we have characterized the small RNAs that associated with BmAGO2 protein in Bombyx mori (PRJNA178305). To characterize targets of small RNAs that associated with BmAGO2 protein in Bombyx mori, the RNA population associated with BmAGO2 in BmN cells was extracted from the AGO immunoprecipitated complex and the RNAs with the length larger than 200nt separated by a polyacrylamide gel electrophoresis were subjected to library construction and deep sequencing. The high throughput sequencing yielded a total of 11,549,040 reads, representing 2,336,881 unique reads with a abundance from 395,989 to 1. Overall design: Long RNA associated with AGO2 of BmN cells infected with ie1-bacmid recombinant viruses were performed by high-throughput using Hiseq 2000

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