A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade

The goal of this project was to assess differential gene expression in the Ventral Nerve Cord (VNC) of adult Drosophila 5 hours after severing of the legs, wings and head. Overall design: Gene expression was assessed in 2 conditions (No Injury and 5-hrs after Injury) in the w1118 strain of Drosophila melanogaster. 5 independent biological replicates were used for each condition. RNA was isolated from the adult Ventral Nerve Cord (VNC) for the gene expression analysis (RNAseq).

41

Purice MD, Ray A, Münzel EJ, Pope BJ, Park DJ, Speese SD, Logan MA