RNA-seq profiles of wildtype and Ezh2 knockout mouse natural killer T cell subsets

Natural killer T (NKT) cells identified by CD1d-tetramer and TCRb were isolated from the thymi of wild type and Ezh2 knockout mice. The NKT cells were FACS sorted into different stages based on the surface expression of CD44 and NK1.1. Overall design: For both wildtype and knockout mice, RNA was extracted from two biological replicates of CD44+ NK1.1- cells, one replicate of CD44+ NK1.1+ cells and one replicate of CD44- NK1.1- cells. Each RNA sample was divided into four and sequenced on four lanes of an Illumina HiSeq sequencer.

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Vasanthakumar A, Xu D, Lun AT, Kueh AJ, van Gisbergen KP, Iannarella N, Li X, Yu L, Wang D, Williams BR, Lee SC, Majewski IJ, Godfrey DI, Smyth GK, Alexander WS, Herold MJ, Kallies A, Nutt SL, Allan RS