Identification of NAD+ capped mRNAs in S. cerevisiae

We report the identification of NAD-RNA in the yeast Saccharomyces cerevisiae under two distinct growth mediums. Overall design: RNA-seq libraries (in triplicate) were generated from total RNA that was treated with ADP-ribosylcyclase to select for NAD-RNAs and compared to mock-treated samples.  After ADPRC treatment, RNA was biotinylated using click chemistry and a pull down using streptavidin beads was performed.

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Walters RW, Matheny T, Mizoue LS, Rao BS, Muhlrad D, Parker R