Schistosoma mansoni strain:LE Transcriptome or Gene expression

Gene silencing generally use a nonrelevant dsRNA from another species as controls and quantitative real time PCR (qPCR) to measure the knockdown levels achieved. Despite the applicability of RNAi to study many genes in schistosomes and others helminthes parasites, several authors have noticed inconsistencies associated with this technique. We used an RNA-seq approach to globally check if there are genes affected by unspecific dsRNA exposure, we cultivated schistosomula and exposed to unspecific dsRNAs synthetized from the Green Fluorescent Protein (GFP) or mCherry, two sequences with no similarity with Schistosoma genome and widely used by the scientific community.

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