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Accession IconSRP093675

Infection of Arabidopsis thaliana seedlings with the Pseudomonas syringae strain DC3000 cor- carrying the effector HopBB1

Organism Icon Arabidopsis thaliana
Sample Icon No Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
This study evaluates the transcriptome of Arabidopsis thaliana infected with the Pseudomonas syringae strain DC3000 cor- carrying the type three secretion system effector HopBB1 Overall design: Two-week-old Arabidopsis thaliana seedlings (Col-0 ecotype) were sprayed with a mock solution (10 mM MgCl2) or bacteria [Pto DC3000 (EV), Pto DC3000 cor- (EV); Pto DC3000 cor- (HopBB1); Pto DC3000 cor- (HopBB1G126D)] at OD600=0.2 with 10mM MgCl2 and 0.04% Silwet L-77. Samples were harvested 24 hours after infection. This experiment included three biological replicates, each one corresponding to approximately 30 seedlings grown in the same pot.
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15
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