Protointrons in Saccharomyces sp.

We sequenced non-ribosomal RNA from a upf1 deletion mutant of S. cerevisiae, and from wild type S. mikatae and S. bayanus, and identified a new class of yeast introns. Overall design: Yeast non-ribosomal RNAs were isolated from vegetatively growing cells or cells treated with rapamycin for 60 minutes to mimick starvation, RNA was sequenced, and splice junctions were identified and validated.

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Talkish J, Igel H, Perriman RJ, Shiue L, Katzman S, Munding EM, Shelansky R, Donohue JP, Ares M Jr