Illumina HiSeq 2500 paired end sequencing; GSM2361351: cd177negtreg_tumor_patient10; Homo sapiens; RNA-Seq

cd177negtreg_tumor_patient10

The goal of this study is to compare transcriptional profiles of regulatory T cells and conventional CD4 T cells in human breast cancer to regulatory T cells and conventional CD4 T cells in normal breast parenchyma and in peripheral blood. Overall design: RNA sequencing of 2 different cell types in 3 different tissues

Regulatory T cells exhibit distinct features in human breast cancer

Submitted by Gene Expression Omnibus on 16-NOV-2016

Illumina TruSeq paired-end library preparation following manufacturer's protocols Cells were sorted directly into Trizol LS (ThermoFisher) and stored at -80Â°C prior to RNA extraction. RNA was extracted and quantified and identical quantities of RNA from each sample were subjected to SMARTer amplification with 12-16 round of PCR was performed.

Regulatory T cells exhibit distinct features in human breast cancer

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