Illumina HiSeq 2000 paired end sequencing; GSM2360631: 4006 RNA-seq; Mus musculus; RNA-Seq

4006 RNA-seq

RNA-seq analysis of 195 randomly selected (TRAMP x J:DO) F1 prostates that had been harvested at experimental termination (210 days or humane endpoints) was performed to identify metastasis associated transcriptomic changes Overall design: Total RNA expression profile of TRAMP x DO F1 prostate tumors

Candidate Gene Identification Through RNA-Seq Analysis of (TRAMP x J:DO) F1 Primary Tumors

Submitted by Gene Expression Omnibus on 02-JAN-2017

total RNA was extracted from snap frozen (TRAMP x J:DO) F1 bulk tumor samples using TRIzol Reagent (Life Technologies, Inc.) according to the manufacturer’s protocol, and purified using RNeasy mini kit (QIAGEN) according to the manufacturer’s protocol. RNA-Seq libraries were constructed from 1 μg total RNA after rRNA depletion using Ribo-Zero GOLD (Illumina). The Illumina TruSeq RNA Sample Prep V2 Kit was used according to manufacturer’s instructions. The cDNAs were fragmented to ~275 bp using a Covaris E210. Libraries were  pooled together for sequencing. The pooled libraries were sequenced on multiple lanes of a HiSeq 2500 using version 4 chemistry to achieve a minimum of 43 million 126 base read pairs

Candidate Gene Identification Through RNA-Seq Analysis of (TRAMP x J:DO) F1 Primary Tumors

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Samples