Approximately 500mg fresh weight of infected and uninfected root tissue was harvested from each of three replicate plants at 3, 6, 9, and 12 days after inoculation (DAI), a total of 24 samples (2 tissue types x 4 dates x 3 biological replicates). Tissue was stored in RNAlater® Stabilization Reagent (Ambion, Austin, TX) and transported to the University of Arizona Genetics Core (Tucson, AZ) for 100-bp paired-end library preparation, barcode tagging, and sequencing on two lanes of the Illumina HiSeq 2000 platform (Illumina, San Diego, CA).  Read quality was assessed with FastQC v0.10.1.  Read were processed by Trimmomatic v0.32 and ConDeTri v2.2.

Illumina HiSeq 2000 paired end sequencing; RNAseq of Glycine max:  DAI6 infected

Gmax_6-IN-3

Reads were obtained from an RNAseq study examining infection of Rotylenchulus reniformis (reniform nematode) on susceptible soybean. The goal of the study was to identify plant genes involved in the formation of the nematode-induced feeding site to better understand the mechanisms by which reniform nematodes parasitize plant roots.

Glycine max cultivar:Hutcheson Raw sequence reads

Submitted by Clemson University on 19-OCT-2016

Approximately 500mg fresh weight of infected and uninfected root tissue was harvested from each of three replicate plants at 3, 6, 9, and 12 days after inoculation (DAI), a total of 24 samples (2 tissue types x 4 dates x 3 biological replicates). Tissue was stored in RNAlaterÂ® Stabilization Reagent (Ambion, Austin, TX) and transported to the University of Arizona Genetics Core (Tucson, AZ) for 100-bp paired-end library preparation, barcode tagging, and sequencing on two lanes of the Illumina HiSeq 2000 platform (Illumina, San Diego, CA).  Read quality was assessed with FastQC v0.10.1.  Read were processed by Trimmomatic v0.32 and ConDeTri v2.2.