Global transcriptome comparison reveals stress adaptation between Russet Norkotah and its Clonal Selection, TXNS278

The Texas Potato breeding program has been succesful at bringing several potato variety including somaclonal selection made in the field. This genetic diversity is in part due to somaclonal variability that appears within potato selections for which tubers are used as seeds. However, nogenetic or molecular evidences were identified so far. In this investigation, we used RNA-seq, which allows genome-wide gene expression analysis to compare the transcriptomes of the subclonal Russet Norkotah selection TXNS278 with standard Russet Norkotah grown in commercial fields. Among the selections, TXNS278 appeared in a multi- year analysis as a top No 1 yielding variety. Russet Norkotah and TXNS278 leaf and root transcriptomes were compared at two time points. Results indicated that yield differences between Russet Norkotah and TXNS278 might possibly be linked to differences in responses to biotic and abiotic stresses and hormonal signaling  Overall design: Texas Somaclonal selection RN and TXNS278 mRNA profiles of 66-day old and 84-day old leaf and root tissue. The plant were grown in the field condition. Sequences were generated by deep sequencing, in triplicate, using Illumina Hiseq-2000 platform and CyVerse.

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Levy J, Tamborindeguy C, Athrey G, Scheuring DC, Koym JW, Miller JC Jr