Comprehensive profiling of mRNA m6A methylome revealed by m6A-seq in porcine adipose and muscle tissues

Using Jinhua and Landrace pigs as fat and lean models, we present a comprehensive transcriptome-wide m6A profiling in adipose and muscle tissues from these two pig breeds.The results show m6A is widely spread and highly conserved in pig mRNA. m6A occurs in the conserved sequence motif of GGACU and exhibits a unique topology in pig. m6A peak enrichment correlates positively with gene expression, and transcripts with m6A at 5'UTR or CDS are correlated with gene activation than those at 3'UTR. Common m6A peaks in both layer of backfat from Landrace (L-LB) and Jinhua (J-LB) pigs are involved in lipid metabolism, while common m6A peaks in both longissimus dorsi muscle from Landrace (L-LDM) and Jinhua (J-LDM) mapped to muscle development, suggesting m6A-containing genes are involved in many biological processes especially related to tissue-specific functions. Overall design: m6A-seq of mRNA from Jinhua LB (J-LB), Jinhua LDM (J-LDM), Landrace LB (L-LB) and Landrace LDM (L-LDM) tissue.

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