Next Generation Sequencing Facilitates identification of genes differentially expressed during feather elongation

Purpose: The goals of this study is to identify genes differentially expressed at different stages of feather elongation Overall design: Hamburger & Hamilton (H&H) stage 31 (Embryonic day 7) and H&H 35 (Embryonic day 9) chicken dorsal skins were dissected out. Epithelium and mesenchyme were separated in 2X CMF. Total RNA was extracted using Trizol reagent. For library construction we used TruSeq RNA Sample Prep Kit Version2 (Illumina). Two biological replicates were sent for sequencing (50bp, single end) with Illumina HiSeq 2000 in the USC Epigenome Center.

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Li A, Cho JH, Reid B, Tseng CC, He L, Tan P, Yeh CY, Wu P, Li Y, Widelitz RB, Zhou Y, Zhao M, Chow RH, Chuong CM