Zea mays Transcriptome or Gene expression

Zea mays B73 seedlings were grown for 3 days, and the terminal 0-1 mm root segments were excised, and snap frozen. Total RNA was extracted from the roots, and contaminating DNA was removed by DNase treatment. Ribosomal RNA was depleted using a Ribo-Zero kit. The rRNA-depleted RNA was used for cDNA conversion and Illumina sequencing to generate whole genome transcriptional activity data.

3