Illumina HiSeq 2000 paired end sequencing; GSM2204137: dry; Glycine max; RNA-Seq

Purpose: This RNA-Seq study aims on elucidate the major trends in the transcriptional profile of soybean embryonic axes during germination. Methods: Soybean seeds were germinated in soaked cotton at 28ºC. In addition to dry seeds, seeds were harvested at 3, 6, 12, 24 hours after imbibition. Then the embryonic axes were separated from the cotyledons for RNA extraction. For each biological sample, 20 seeds were used. Results:  Identification of genes and pathways involved in metabolism, hormone signaling and transcriptional regulation. Overall design: Large-scale analysis of soybean embryonic axis in five time points during germination: dry, 3, 6, 12 and 24 hours after imbibition.

Large-scale analysis of soybean embryonic axis in five time points during germination

Submitted by Gene Expression Omnibus on 15-FEB-2017

Purpose: This RNA-Seq study aims on elucidate the major trends in the transcriptional profile of soybean embryonic axes during germination. Methods: Soybean seeds were germinated in soaked cotton at 28ÂºC. In addition to dry seeds, seeds were harvested at 3, 6, 12, 24 hours after imbibition. Then the embryonic axes were separated from the cotyledons for RNA extraction. For each biological sample, 20 seeds were used. Results:  Identification of genes and pathways involved in metabolism, hormone signaling and transcriptional regulation. Overall design: Large-scale analysis of soybean embryonic axis in five time points during germination: dry, 3, 6, 12 and 24 hours after imbibition.

For RNA extraction, embryonic axes were immediately placed in RNAlater (Qiagen) until RNA extraction. Total RNA was isolated using RNeasy plant mini kits (Qiagen). RNA-Seq libraries were prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina, cat. RS-122-2001) and submitted to paired-end sequencing (2x100bp) using a HiSeq 2000 instrument